Journal:

Article Title: Differential effect on TCR:CD3 stimulation of a 90-kD glycoprotein (gp90/Mac-2BP), a member of the scavenger receptor cysteine-rich domain protein family

doi: 10.1046/j.1365-2249.1998.00654.x

Figure Lengend Snippet: Effect of gp90/Mac-2BP on the lymphocyte proliferative response to different anti-TCR:CD3 MoAbs. Freshly isolated peripheral blood mononuclear cells (PBMC) were cultured in the presence and the absence of gp90/Mac-2BP (100 μg/ml), and stimulated with anti-CD3 (OKT3, CBT3) or anti-TCR αβ (BMA-031, WT-31) MoAbs, as detailed in Materials and Methods. Results were expressed as percent response in the presence of gp90/Mac-2BP (▪) compared with the results obtained in its absence (□, = 100%). Mean values ± 1 s.d. of 36 consecutive experiments are reported.

Article Snippet: The anti-TCR MoAbs used were WT-31 (IgG1; Becton Dickinson, San José, CA) and BMA-031 (IgG2b; Behringwerke AG, Marburg/Lahn, Germany).

Techniques: Isolation, Cell Culture

Journal:

Article Title: Differential effect on TCR:CD3 stimulation of a 90-kD glycoprotein (gp90/Mac-2BP), a member of the scavenger receptor cysteine-rich domain protein family

doi: 10.1046/j.1365-2249.1998.00654.x

Figure Lengend Snippet: Effect of gp90/Mac-2BP on the proliferative response of T cell blasts to solid-phase MoAbs directed against the TCR:CD3 complex*

Article Snippet: The anti-TCR MoAbs used were WT-31 (IgG1; Becton Dickinson, San José, CA) and BMA-031 (IgG2b; Behringwerke AG, Marburg/Lahn, Germany).

Techniques:

Journal:

Article Title: Differential effect on TCR:CD3 stimulation of a 90-kD glycoprotein (gp90/Mac-2BP), a member of the scavenger receptor cysteine-rich domain protein family

doi: 10.1046/j.1365-2249.1998.00654.x

Figure Lengend Snippet: Effect of anti-CD5 MoAb on the lymphocyte proliferative response to anti-TCR:CD3 MoAbs. Freshly isolated peripheral blood mononuclear cells (PBMC) were cultured in the presence of different anti-CD5 MoAb concentrations, and stimulated with anti-CD3 (CBT3, ▪) or anti-TCR αβ (BMA-031, •) MoAb, ▴, Lymphocyte response to anti-CD5 MoAb in the absence of anti-TCR:CD3 MoAbs. Results were expressed as ct/min of 3H-TdR incorporation; one representative experiment of four consecutive is reported.

Article Snippet: The anti-TCR MoAbs used were WT-31 (IgG1; Becton Dickinson, San José, CA) and BMA-031 (IgG2b; Behringwerke AG, Marburg/Lahn, Germany).

Techniques: Isolation, Cell Culture